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antibodies against spp1  (Proteintech)


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    Structured Review

    Proteintech antibodies against spp1
    Antibodies Against Spp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+spp1/Osteopontin+Antibody/pm41899624-79-5-9
    Average 96 stars, based on 636 article reviews
    antibodies against spp1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Identification of diagnostic biomarkers and immune cell infiltration in tongue squamous cell carcinoma using bioinformatic approaches
    Article Snippet: .. They were then incubated overnight at 4 °C with primary antibodies against SPP1 (1:50, Proteintech, 22952-1-AP) and CD68 (1:100, Invitrogen, 14-0688-82). .. After washing the tissue sections three times in PBS (pH 7.2), secondary antibodies, such as goat anti-rabbit conjugated with Alexa Fluor 594 and goat anti-mouse conjugated with Alexa Fluor 488 (ZSGB-Bio), were applied for 1 h at 37 °C.

    Article Title: Lung Adenocarcinoma Promotes NETosis via the NPM1-TNFAIP6-CD44-SPP1 Axis.
    Article Snippet: To standardize total protein input across samples, cells were trypsinized and counted using a TC20 automated cell counter (Bio-Rad, Hercules, CA, USA), and the volume of RIPA lysis buffer was adjusted accordingly. .. Cell lysates were incubated with antibodies against SPP1 (22952-1-AP, Proteintech), TNFAIP6 (ab267469, Abcam, Cambridge, UK), CD44 (15675-1-AP, Proteintech), or normal IgG (Beyotime, Shanghai, China) at 4 ◦C for 24 h. Protein A/G agarose beads (Beyotime) were then added, and the samples were gently rotated for an additional 3 h at 4 ◦C. ..

    Article Title: Lung Adenocarcinoma Promotes NETosis via the NPM1–TNFAIP6–CD44–SPP1 Axis
    Article Snippet: To standardize total protein input across samples, cells were trypsinized and counted using a TC20 automated cell counter (Bio-Rad, Hercules, CA, USA), and the volume of RIPA lysis buffer was adjusted accordingly. .. Cell lysates were incubated with antibodies against SPP1 (22952-1-AP, Proteintech), TNFAIP6 (ab267469, Abcam, Cambridge, UK), CD44 (15675-1-AP, Proteintech), or normal IgG (Beyotime, Shanghai, China) at 4 °C for 24 h. Protein A/G agarose beads (Beyotime) were then added, and the samples were gently rotated for an additional 3 h at 4 °C. ..

    Article Title: Identification of diagnostic biomarkers and immune cell infiltration in tongue squamous cell carcinoma using bioinformatic approaches.
    Article Snippet: .. They were then incubated overnight at 4 °C with primary antibodies against SPP1 (1:50, Proteintech, 22952-1-AP) and CD68 (1:100, Invitrogen, 14-0688-82). .. After washing the tissue sections three times in PBS (pH 7.2), secondary antibodies, such as goat anti-rabbit conjugated with Alexa Fluor 594 and goat anti-mouse conjugated with Alexa Fluor 488 (ZSGBBio), were applied for 1 h at 37 °C.

    other:

    Article Title: EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer
    Article Snippet: We purchased SYBR Green, FBS, RPMI 1640, Alexa Fluor–tagged secondary antibodies, and DAPI from Life Technologies (Thermo Fisher Scientific); paraformaldehyde from Electron Microscopy Sciences; puromycin from InvivoGen; G418 from Corning; brefeldin A (B7651) from MilliporeSigma; murine (TRCN0000025579, TRCN0000025580, and TRCN0000025582) and human (TRCN0000196550) PI4K2A shRNAs from MilliporeSigma; PI4KB (SASI_Hs01_00149544),PI4K2A (SASI_Hs01_00190417 and SASI_Hs01_00190418), ZEB1 (SASI_Hs02_00330526 and SASI_Hs02_00330527), SNAI1 (SASI_Hs01_00039785 and SASI_Hs01_00039786), SNAI2 (SASI_Hs01_00159363 and SASI_Hs01_00159364) siRNAs from MilliporeSigma; antibodies against α-tubulin (no. T9026), Flag tag (no. F3165), MYC tag (no. 05-724), or EGFP (no. G6539) from MilliporeSigma; antibodies against PARP-1 (no. 9542), HA-tag (nos.

    Article Title: TAMs-derived SPP1, regulated by HIF-1α/STAT3 signaling pathway, influences colorectal cancer malignant progression by activation of EMT via integrin αvβ3.
    Article Snippet: Liver metastasis of colorectal cancer (CRC) is characterized by a high recurrence rate after surgery, which may be related to the rerecruitment of residual tumor cells by other factors that promote cancer cell growth in the tumor microenvironment.. Tumor-associated macrophages (TAMs), as key immune components, showed high expression of secretory phosphoprotein-1 (SPP1) at the site of liver metastasis in colorectal cancer patients.. However, the factors and mechanisms driving the elevated expression of SPP1 in TAMs remain poorly understood, as do the potential effects of SPP1 on colorectal cancer progression.

    Recombinant:

    Article Title: EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer
    Article Snippet: 2023;133(7):e165863 https://doi.org/10.1172/JCI165863 shRNAs from MilliporeSigma; PI4KB (SASI_Hs01_00149544),PI4K2A (SASI_Hs01_00190417 and SASI_Hs01_00190418), ZEB1 (SASI_ Hs02_00330526 and SASI_Hs02_00330527), SNAI1 (SASI_Hs01_ 00039785 and SASI_Hs01_00039786), SNAI2 (SASI_Hs01_00159363 and SASI_Hs01_00159364) siRNAs from MilliporeSigma; antibodies against α-tubulin (no. T9026), Flag tag (no. F3165), MYC tag (no. 05-724), or EGFP (no. G6539) from MilliporeSigma; antibodies against PARP-1 (no. 9542), HA-tag (nos. .. 3724 and 2367), β-actin (no. 4970), GM130 (no. 12480), Golgin-97 (no. 13192), AXL (no. 8661), and EGFR (no. 2256) from Cell Signaling Technology; antibodies against ACBD3 (sc-101277), ZEB1 (sc-25388), and PI4K2A (sc-390026) from Santa Cruz Biotechnology; antibodies against SPP1 (22952-1-AP), SEMA7A (18070-1-AP), HSP90 (11405-1-AP), ZFP36L1 (12306-1-AP), and CD44 (15675-1-AP) from Proteintech; antibodies against ITGB1 (GTX636657) from GeneTex; antibodies against RNA polymerase II (AB_2732926) from Active Motif; recombinant SPP1 proteins (ab92964) from Abcam; recombinant Gas6 (885-GSB) and SPP1 (1433-OP-050) proteins from R&D Systems; PI-273 (HY-103489) from MedChemExpress; NC03 (AOB17420) from Aobious; and miRNA mimics (HMI0508, HMI0275, and HMI0280) and control mimics (HMC0002) from MilliporeSigma. ..

    Control:

    Article Title: EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer
    Article Snippet: 2023;133(7):e165863 https://doi.org/10.1172/JCI165863 shRNAs from MilliporeSigma; PI4KB (SASI_Hs01_00149544),PI4K2A (SASI_Hs01_00190417 and SASI_Hs01_00190418), ZEB1 (SASI_ Hs02_00330526 and SASI_Hs02_00330527), SNAI1 (SASI_Hs01_ 00039785 and SASI_Hs01_00039786), SNAI2 (SASI_Hs01_00159363 and SASI_Hs01_00159364) siRNAs from MilliporeSigma; antibodies against α-tubulin (no. T9026), Flag tag (no. F3165), MYC tag (no. 05-724), or EGFP (no. G6539) from MilliporeSigma; antibodies against PARP-1 (no. 9542), HA-tag (nos. .. 3724 and 2367), β-actin (no. 4970), GM130 (no. 12480), Golgin-97 (no. 13192), AXL (no. 8661), and EGFR (no. 2256) from Cell Signaling Technology; antibodies against ACBD3 (sc-101277), ZEB1 (sc-25388), and PI4K2A (sc-390026) from Santa Cruz Biotechnology; antibodies against SPP1 (22952-1-AP), SEMA7A (18070-1-AP), HSP90 (11405-1-AP), ZFP36L1 (12306-1-AP), and CD44 (15675-1-AP) from Proteintech; antibodies against ITGB1 (GTX636657) from GeneTex; antibodies against RNA polymerase II (AB_2732926) from Active Motif; recombinant SPP1 proteins (ab92964) from Abcam; recombinant Gas6 (885-GSB) and SPP1 (1433-OP-050) proteins from R&D Systems; PI-273 (HY-103489) from MedChemExpress; NC03 (AOB17420) from Aobious; and miRNA mimics (HMI0508, HMI0275, and HMI0280) and control mimics (HMC0002) from MilliporeSigma. ..



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    Differential gene expression of ICC tumor cells. (A) Normal liver cells and tumor cells isolated from 4 patients with ICC utilizing a graph-based Louvain clustering algorithm on the K-Nearest Neighbors graph. (B) Specific marker gene expression of four tumors and normal liver cells. (C) The four ICC tumor cells were classified into specific CNV groups based on their identified CNV patterns. (D) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed genes of ICC tumor cells. The circle size represents the level of statistical significance, and the circle color represents the intensity of the interaction. (E) Volcano plot displaying the 176 significantly upregulated and 228 downregulated genes. (F) <t>SPP1</t> is overexpressed in ICC tumor cells. (G) SPP1 upregulation was observed in ICC tumors when compared with both non-tumor tissues and liver tumors based on data obtained from the Metabolic gEne RApid Visualizer website. (H) Increased expression levels of SPP1 are correlated with a poor prognosis for patients with ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; CNV, copy number variant; NLT, normal liver tissue; HCC, hepatocellular carcinoma; HR, hazard ratio; UMAP, Uniform Manifold Approximation and Projection.
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    Differential gene expression of ICC tumor cells. (A) Normal liver cells and tumor cells isolated from 4 patients with ICC utilizing a graph-based Louvain clustering algorithm on the K-Nearest Neighbors graph. (B) Specific marker gene expression of four tumors and normal liver cells. (C) The four ICC tumor cells were classified into specific CNV groups based on their identified CNV patterns. (D) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed genes of ICC tumor cells. The circle size represents the level of statistical significance, and the circle color represents the intensity of the interaction. (E) Volcano plot displaying the 176 significantly upregulated and 228 downregulated genes. (F) <t>SPP1</t> is overexpressed in ICC tumor cells. (G) SPP1 upregulation was observed in ICC tumors when compared with both non-tumor tissues and liver tumors based on data obtained from the Metabolic gEne RApid Visualizer website. (H) Increased expression levels of SPP1 are correlated with a poor prognosis for patients with ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; CNV, copy number variant; NLT, normal liver tissue; HCC, hepatocellular carcinoma; HR, hazard ratio; UMAP, Uniform Manifold Approximation and Projection.
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    Image Search Results


    Differential gene expression of ICC tumor cells. (A) Normal liver cells and tumor cells isolated from 4 patients with ICC utilizing a graph-based Louvain clustering algorithm on the K-Nearest Neighbors graph. (B) Specific marker gene expression of four tumors and normal liver cells. (C) The four ICC tumor cells were classified into specific CNV groups based on their identified CNV patterns. (D) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed genes of ICC tumor cells. The circle size represents the level of statistical significance, and the circle color represents the intensity of the interaction. (E) Volcano plot displaying the 176 significantly upregulated and 228 downregulated genes. (F) SPP1 is overexpressed in ICC tumor cells. (G) SPP1 upregulation was observed in ICC tumors when compared with both non-tumor tissues and liver tumors based on data obtained from the Metabolic gEne RApid Visualizer website. (H) Increased expression levels of SPP1 are correlated with a poor prognosis for patients with ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; CNV, copy number variant; NLT, normal liver tissue; HCC, hepatocellular carcinoma; HR, hazard ratio; UMAP, Uniform Manifold Approximation and Projection.

    Journal: Oncology Letters

    Article Title: Single-cell RNA sequencing analysis of intrahepatic cholangiocarcinoma reveals SPP1 facilitates disease progression via interaction with CD4 + T cells

    doi: 10.3892/ol.2025.15135

    Figure Lengend Snippet: Differential gene expression of ICC tumor cells. (A) Normal liver cells and tumor cells isolated from 4 patients with ICC utilizing a graph-based Louvain clustering algorithm on the K-Nearest Neighbors graph. (B) Specific marker gene expression of four tumors and normal liver cells. (C) The four ICC tumor cells were classified into specific CNV groups based on their identified CNV patterns. (D) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed genes of ICC tumor cells. The circle size represents the level of statistical significance, and the circle color represents the intensity of the interaction. (E) Volcano plot displaying the 176 significantly upregulated and 228 downregulated genes. (F) SPP1 is overexpressed in ICC tumor cells. (G) SPP1 upregulation was observed in ICC tumors when compared with both non-tumor tissues and liver tumors based on data obtained from the Metabolic gEne RApid Visualizer website. (H) Increased expression levels of SPP1 are correlated with a poor prognosis for patients with ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; CNV, copy number variant; NLT, normal liver tissue; HCC, hepatocellular carcinoma; HR, hazard ratio; UMAP, Uniform Manifold Approximation and Projection.

    Article Snippet: Subsequently, the tissues were blocked with 3% BSA in PBS for 30 min at room temperature and incubated overnight at 4°C with antibody against human SPP1 (1:100; cat. no. ER1802-16; Hangzhou HuaAn Biotechnology Co., Ltd.).

    Techniques: Gene Expression, Isolation, Marker, Expressing, Variant Assay

    Distinguishing T-cell subsets in ICC tumor tissues between the early and late stages of disease. (A) Identification of ICC tumor cells in the early and late stage of disease. (B) Expression of SPP1 is higher in late-stage ICC tumor tissues compared with early-stage tumor tissues. ***P<0.001. (C) A total of seven subsets of T cells in the late stages were identified utilizing a graph-based Louvain clustering algorithm applied to the KNN graph. (D) A total of seven subsets of T cells in the early stages were identified utilizing a graph-based Louvain clustering algorithm applied to the KNN graph. (E) Ratio of Treg cells to Tcm cells in early- and late-stage ICC. (F) Pseudotime analysis of T-cell diffusion patterns across various stages of ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; KNN, K-Nearest Neighbors; Treg, regulatory T cells; Tcm, central memory T cells; UMAP, Uniform Manifold Approximation and Projection.

    Journal: Oncology Letters

    Article Title: Single-cell RNA sequencing analysis of intrahepatic cholangiocarcinoma reveals SPP1 facilitates disease progression via interaction with CD4 + T cells

    doi: 10.3892/ol.2025.15135

    Figure Lengend Snippet: Distinguishing T-cell subsets in ICC tumor tissues between the early and late stages of disease. (A) Identification of ICC tumor cells in the early and late stage of disease. (B) Expression of SPP1 is higher in late-stage ICC tumor tissues compared with early-stage tumor tissues. ***P<0.001. (C) A total of seven subsets of T cells in the late stages were identified utilizing a graph-based Louvain clustering algorithm applied to the KNN graph. (D) A total of seven subsets of T cells in the early stages were identified utilizing a graph-based Louvain clustering algorithm applied to the KNN graph. (E) Ratio of Treg cells to Tcm cells in early- and late-stage ICC. (F) Pseudotime analysis of T-cell diffusion patterns across various stages of ICC. ICC, intrahepatic cholangiocarcinoma; SPP1, secreted phosphoprotein 1; KNN, K-Nearest Neighbors; Treg, regulatory T cells; Tcm, central memory T cells; UMAP, Uniform Manifold Approximation and Projection.

    Article Snippet: Subsequently, the tissues were blocked with 3% BSA in PBS for 30 min at room temperature and incubated overnight at 4°C with antibody against human SPP1 (1:100; cat. no. ER1802-16; Hangzhou HuaAn Biotechnology Co., Ltd.).

    Techniques: Expressing, Diffusion-based Assay

    Interactions between tumor cells and different immune cell types. (A) Bubble plot representing ligand-receptor interactions between tumor cells and immune cell clusters in ICC. (B) Details of interaction between tumor cells and immune cells in ligand-receptor pair of SPP1-CD44. (C) Details of interaction between tumor cells and immune cells in ligand-receptor pair of MIF-(CD74+CXCR4). (D) Details of interaction between tumor cells and immune cells in ligand-receptor pair of CXCL12-CXCR4. (E) Details of interaction between tumor cells and immune cells in ligand-receptor pair of CCL3-CCR1. ICC, intrahepatic cholangiocarcinoma; MIF, macrophage migration inhibitory factor; SPP1, secreted phosphoprotein 1; CXCR4, C-X-C chemokine receptor type 4; CCR4, C-C chemokine receptor type 1; CCL3, C-C motif chemokine ligand 3; DC, dendritic cell; NK, natural killer.

    Journal: Oncology Letters

    Article Title: Single-cell RNA sequencing analysis of intrahepatic cholangiocarcinoma reveals SPP1 facilitates disease progression via interaction with CD4 + T cells

    doi: 10.3892/ol.2025.15135

    Figure Lengend Snippet: Interactions between tumor cells and different immune cell types. (A) Bubble plot representing ligand-receptor interactions between tumor cells and immune cell clusters in ICC. (B) Details of interaction between tumor cells and immune cells in ligand-receptor pair of SPP1-CD44. (C) Details of interaction between tumor cells and immune cells in ligand-receptor pair of MIF-(CD74+CXCR4). (D) Details of interaction between tumor cells and immune cells in ligand-receptor pair of CXCL12-CXCR4. (E) Details of interaction between tumor cells and immune cells in ligand-receptor pair of CCL3-CCR1. ICC, intrahepatic cholangiocarcinoma; MIF, macrophage migration inhibitory factor; SPP1, secreted phosphoprotein 1; CXCR4, C-X-C chemokine receptor type 4; CCR4, C-C chemokine receptor type 1; CCL3, C-C motif chemokine ligand 3; DC, dendritic cell; NK, natural killer.

    Article Snippet: Subsequently, the tissues were blocked with 3% BSA in PBS for 30 min at room temperature and incubated overnight at 4°C with antibody against human SPP1 (1:100; cat. no. ER1802-16; Hangzhou HuaAn Biotechnology Co., Ltd.).

    Techniques: Migration